FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3024_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3024_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159241
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3280.20597710388656187No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3230.20283720901024235No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT2730.17143826024704692No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA2620.16453049151914392No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2290.14380718533543496No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2110.13250356378068462No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA2080.1306196268548929No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1860.11680408939908692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGC100.007233469143.99978
CGTCGCT100.007233469143.99979
GAGCGAA1150.093.9423451
GAGCAAA5050.082.7188641
CGTTACA651.6407284E-977.538293
GCAAAAG5500.075.927113
AGCGAAA1400.071.999852
ACCGATA300.001986554671.999848
AGCAAAA5950.071.3948062
GCGAAAG1750.061.714153
CAAAAGC6850.060.963374
GTTACAC851.3687895E-859.2939834
CGAAAGC1950.055.384494
AAAGCAG9600.054.749886
GAACGGA400.00620931553.9998866
TTACACC953.291825E-853.0525175
AAAAGCA8350.050.874145
ATCTAGA951.9995987E-645.4878731
ACACCTT1151.481003E-743.8259937
CAGCTCG1151.481003E-743.8259939