FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3022_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3022_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88450
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2050.2317693612210288No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT1860.21028829847371394No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1810.20463538722442057No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1490.1684567552289429No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1240.14019219898247598No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG980.11079706048615037No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT960.10853589598643301No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT960.10853589598643301No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG930.10514414923685697No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC900.10175240248728094No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA3150.0107.6089551
GAGCGAA554.274625E-1091.790231
AGCAAAA4200.080.615422
GCAAAAG4250.077.9719543
CAAAAGC4400.075.2712554
AGCGAAA702.917659E-972.039312
GCGAAAG755.0349627E-967.236693
AAAAGCA5350.061.9053275
ATTATAT350.003646695861.747983
AAAGCAG6200.061.5471766
CTATCCC400.00620065653.9989434
GCAGGTA701.7539081E-551.4275639
AAGCAGG7650.050.8225337
TAACCTG605.0918147E-447.9990585
ATAACCT605.0918147E-447.9990584
CGAAAGC1057.1933755E-847.9990544
ACAGTAT657.554087E-444.3068248
AGCAGGT2650.040.7539148
GCAGGGG3550.036.5063259
AGCAGGG5800.035.9992948