FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_3021_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_3021_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145144
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT5380.3706663727057267No Hit
GGCTGCTGCCTGTGGTGAAGGCTCATCAGGGAGGAGATAGGGCGAAGGGA3190.2197817340020945No Hit
GCTTCTGTCTTTGGCTCAGGTCATGATCCCAGGATCCTGGGATCAAGTCC2930.2018684892244943No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT2830.19497877969464808No Hit
CCCACTTCCCCTGCTTTTGCTCCCCCCAGGGGACCCGGACAGGTCCAGCC2330.16053023204541694No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA2090.14399492917378603No Hit
CCCACTTCCCCTGCTTTCGCTCCCCCCAGGGGACCCGGACAGGTCCAGCC1960.13503830678498596No Hit
TGGAGGAGGGCAAGGGTTAGGTCCAAGTGGAATAGTGGCAGGGTGAGTCC1930.13297139392603208No Hit
CATGGAGGAGGGCAAGGGTTAGGTCCAAGTGGAATAGTGGCAGGGTGAGT1740.11988094581932426No Hit
CTCCCACTTCCCCTGCTTTTGCTCCCCCCAGGGGACCCGGACAGGTCCAG1710.1178140329603704No Hit
TGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA1680.11574712010141651No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA3600.094.0164261
GCAAAAG4750.072.7706153
CAAAAGC4850.072.7549744
AGCAAAA4900.067.603652
GTCTATC350.003656783461.7250752
TCTACTA350.003656783461.7250755
GGTCTAT400.006203864754.0094341
AAAAGCA7100.048.6845635
AGCGAAA1800.048.008391
TACTATA450.00988252848.008392
TATCCCG450.00988252848.008395
CCGACCA450.00989599847.9918489
GTAGAAA2050.045.6665151
GCGAAAG1900.045.481632
TAGAAAC2200.042.5528872
CGAAAGC2050.042.153713
TATAGAC2050.042.153712
AAAGCAG9150.040.9251826
TAGACAT2300.037.571784
ATAGACA2350.036.7723853
Produced by FastQC (version 0.11.2)