FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_2043_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_2043_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361015
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA19530.5409747517416174No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG15700.43488497707851476No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG11700.32408625680373393No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT7680.21273354292757918No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT6330.17533897483484065No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT6240.1728460036286581No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA5770.15982715399637135No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG5340.1479162915668324No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4780.1324044707283631No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT4540.12575654751187626No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA4320.11966261789676329No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA4210.11661565308920682No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT4180.11578466268714596No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC4100.11356868828165034No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT4030.11162971067684169No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4020.11135271387615474No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA3990.11052172347409389No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT3990.11052172347409389No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC3970.10996772987271998No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG3820.10581277786241569No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG3740.10359680345692007No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3730.10331980665623314No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT3650.1011038322507375No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTAT100.007238172143.99354
GAGCGAA1400.0128.583441
TGATAGG259.645076E-486.3960953
CCTACTA608.749339E-1083.996215
CCACCTA608.749339E-1083.996212
GCAGGGT5650.082.828129
GCAGGTA3500.082.2829
AGCGAAA2250.079.996382
CACCTAC651.6498234E-977.534963
TTATAGG1500.076.807171
CGAAAGC2500.071.996754
GCGAAAG2550.070.5850453
AGTAGAA1350.069.3398061
GCAAAAG13700.067.792563
AGCAGGG8650.067.4189158
GATACTT554.2275587E-665.451593
AAGCAGG18000.062.397187
GAGCAAA14700.062.2098851
GTAGAAA2600.060.928761
TAGATAC607.0775804E-660.00561
Produced by FastQC (version 0.11.2)