FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_2041_d3_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_2041_d3_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222832
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG12380.5555755008257341No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA11840.5313419975586989No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG7150.32086953399870755No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA3840.1723271343433618No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT3780.1696345228692468No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3780.1696345228692468No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3710.16649314281611258No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT3520.15796653981474834No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3470.1557226969196525No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3060.13732318517986644No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT3010.1350793422847706No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC2890.12969411933654054No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC2860.12834781359948302No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2850.12789904502046387No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG2630.11802613628204207No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC2610.11712859912400372No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2570.11533352480792705No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC2530.11353845049185037No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG2470.11084583901773534No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC2450.10994830185969699No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2440.10949953328067781No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG2430.10905076470165864No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2400.10770445896460114No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA2380.10680692180656279No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA2350.10546061606950528No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC2310.10366554175342858No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT2310.10366554175342858No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT2280.10231923601637108No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC2260.10142169885833273No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGA100.007236428143.992811
GAGCGAA1400.0128.565021
TTATAGG352.871475E-7102.852021
GCAGGGT5450.091.151419
GCAGGTA2000.086.39579
AGCGAAA2150.083.716772
CCTACTA950.083.3642655
GCGAAAG2200.081.8140953
TCCACCT900.079.996021
CGAAAGC2300.078.2569664
CCACCTA950.075.78572
AGCAGGG8050.073.337958
GCAAAAG10500.071.996413
GAGCAAA11000.069.378361
AAGCAGG14000.067.882337
CACCTAC1100.065.451283
GTGTTTT1001.0913936E-1164.796779
AAAGCAG14800.063.240096
GTAGAAA1600.062.9968571
CAAAAGC12850.058.829754
Produced by FastQC (version 0.11.2)