FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_2039_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_2039_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255615
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10100.395125481681435No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA8460.3309664925767267No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG7820.3059288382919625No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG5520.21594976820609119No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT4780.18699998043933258No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT4640.18152299356454044No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3240.12675312481661874No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT3220.12597069812021988No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3170.12401463137922265No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3050.11932007120082937No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2960.11579915106703441No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA2930.11462551102243608No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2880.11266944428143888No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA2770.10836609745124504No Hit
GAGCAAAAGCAGGTAGACATTTAAAGATGAGTCTTCTAACCGAGGTCGAA2730.10680124405844726No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2720.10641003071024784No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2660.1040627506210512No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG2560.10015061713905679No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA850.0118.6101461
GCAGGTA3050.092.0645759
CACCTAC406.361497E-789.999023
CCTACTA451.2820219E-679.999135
CCACCTA451.2820219E-679.999132
TTATAGG1050.075.442511
TATCCAC406.886148E-571.9992144
GCAGGGT3250.070.891549
AGCGAAA1600.067.5124741
TGCCATG1600.067.499278
GTGTTTT651.4623947E-766.4608159
CTACACT1200.065.999284
GCGAAAG1650.065.4538352
CGAAAGC1700.063.5287253
TCTACAC1250.063.359313
GCAAAAG10050.063.044093
CAGGTAC350.003662666361.713613
TCCACCT607.0678525E-660.011091
GAGCAAA10850.059.0708241
AAGCAGG12900.057.4877477
Produced by FastQC (version 0.11.2)