FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_2033_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_2033_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216076
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA10680.49427053444158536No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG9700.4489161221051852No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG6500.30082008182306225No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG5950.2753660748995724No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT4240.19622725337381294No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT4140.19159925211499657No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT3270.1513356411632944No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC3100.14346803902330663No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3070.14207963864566173No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3020.13976563801625355No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT2830.1309724356245025No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA2580.11940243247746164No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2510.11616283159629019No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2510.11616283159629019No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2410.11153483033747384No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA2380.11014642995982894No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2320.10736962920453914No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2320.10736962920453914No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG2300.10644402895277588No Hit
AGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTAT2290.10598122882689424No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT2280.10551842870101259No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG2270.10505562857513098No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC2240.10366722819748608No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC2210.10227882781984117No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT2200.10181602769395953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA600.0144.03311
CCTACTA650.099.6921545
CACCTAC650.099.6921543
CCACCTA650.099.6921542
TCCACCT700.092.592711
CGAAAGC1650.091.6362153
GCAGGTA1650.091.6362159
AGCGAAA1750.086.419861
GCGAAAG1750.086.399862
GCAGGGT4300.075.348719
TCTACAC1100.071.9998863
CTACACT1100.071.9998864
GCAAAAG9100.071.999883
TTATAGG955.456968E-1268.2262041
GAGCAAA9750.067.215451
AAGCAGG11850.063.189777
GTAGAAA1550.060.4009781
GGCCAAC753.9246152E-757.6132431
AGCAGGG7400.057.405318
AGCAAAA11550.056.727182
Produced by FastQC (version 0.11.2)