FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_2032_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_2032_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365711
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA7760.21218940638919803No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT5860.16023581461864697No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG5790.15832173492183718No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3150.077.71141
CGAAAGC3450.075.1276553
GCGAAAG3700.070.051462
GAGCAAA6350.069.1627961
GCGTATA350.003664307361.7122
CATATAC1101.7771526E-952.3616943
GAGCGAA1650.052.3616941
GCAAAAG9100.048.2619483
CAAAAGC8850.047.9982224
AGCAAAA10350.042.433212
TATAGAC4550.037.9766162
CGTATAC1604.865069E-835.998673
ATAGACA4900.035.2643
AAAAGCA12350.033.229545
ATATACC1204.2216713E-429.9988884
CATCTTA5650.029.308658
TAGACAT5950.027.8309024
ACATCTT6400.026.9997
AAAGCAG19150.026.693536
TCTAGGG1450.001275652724.8266683
Produced by FastQC (version 0.11.2)