FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_2031_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_2031_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255149
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA5060.19831549408384905No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG4830.1893011534436741No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1800.084.028141
GAGCGAA1000.079.226531
GCGAAAG2250.067.196152
CGAAAGC2300.065.7353743
GAGCAAA4500.062.4209021
TTAGACC752.6523738E-547.9972533
CATACAT3600.045.997378
TACATAC3950.040.0989766
GCAAAAG7050.039.827513
CAAAAGC7300.038.4635544
AGCAAAA7350.038.20192
CAGCTCG2551.8189894E-1233.8804179
TATACAT5000.033.1181074
ACATACA5250.031.5410527
TATAGAC1652.510169E-630.5437092
TAGACCT950.00488465230.3140564
GTAGGAT1204.2186255E-429.9982858
TATACAG3700.029.187525
GTATATA7000.028.8096491
GTATTAC1255.345304E-428.8096471
Produced by FastQC (version 0.11.2)