FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_2030_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_2030_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148335
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1510.10179660902686485No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT100.0072336346143.995298
AGCGAAA1350.079.9973751
CGGACCA300.001986526271.997649
CGAAAGC1500.071.9976353
GAGCGAA607.062379E-659.9980351
GCGAAAG1950.055.3827972
TATAGAC1152.6157068E-950.0853162
GTACACC752.646575E-547.9984254
ATAGACC752.646575E-547.9984253
TAGACCT700.001090398341.141514
ATAGACA1001.4435922E-435.998823
GTATAGA2650.035.31961
GTACCTA950.004877547730.3147966
AACCTTT1204.2095943E-429.9990186
GTACCAT1000.00626732728.7990576
TCTAGAC1000.00626732728.7990573
TATGCAG1050.00795295127.4276735
ACTGTAC1050.00795295127.4276733
TGTACAC1358.383782E-426.665793
TAGACAT1658.7766006E-526.180964
Produced by FastQC (version 0.11.2)