FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n01_2029_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n01_2029_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479402
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA10150.21172210378763542No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG9700.20233540953104073No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT7480.1560277178651737No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA6860.14309493911164325No Hit
GGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCTCGGAGCAGGAA5700.11889812725019921No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT5670.1182723476330929No Hit
CCCCCAAAGACCAGAAAATTGCTCCAGGTCCAACTGCCCAGATCCTTGGC4980.10387941643964774No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4500.083.196331
CGAAAGC5050.075.561023
GCGAAAG5200.071.9968262
TATACCG300.001989333471.9968265
ATACCGC300.001989333471.9968266
GAGCAAA8450.064.754531
CGGCTAG350.00366510661.711568
GCAAAAG10650.051.378013
GAGCGAA2450.047.018331
AGCAAAA12850.042.5817762
CAAAAGC14050.040.48224
GCCTTAC907.789876E-539.998231
CAGCTCG4200.035.998419
GGAGCGA1851.7439197E-731.1337591
AAAAGCA18550.031.049845
TATACAG6500.031.0140155
TACCGCT950.004888581630.314457
TACAGCT6950.027.9699887
GTCTTAG1555.761537E-527.8697361
AAAGCAG24600.027.2183116