FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n02_ah8868b-1217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n02_ah8868b-1217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14887585
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC494930.33244478536982325Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG212660.1428438527806894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC62950.038.44138744
TGGTCGC66550.036.3619143
CTCGGTG65450.035.92877638
GTGGTCG67700.035.8089942
ATCTCGG68900.034.3830636
GATCTCG70800.033.18140835
CGTGTAG77050.032.40043616
GTAGGGA77400.032.37009419
GGTGGTC75100.032.25124741
GTGTAGG78350.031.91887917
AGTGTAG78200.031.8142329
TCGGTGG76050.031.76148639
GTCGTGT80100.031.55019414
TCTCGGT75750.031.3021437
GCGTCGT81250.031.05586212
TGTAGGG80900.030.9390418
GAGTGTA80200.030.8834128
AGCGTCG82250.030.70853811
CGTCGTG82650.030.68590413
GAGCGTC84800.029.81115310
Produced by FastQC (version 0.11.2)