FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n02_ah8868b-031517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n02_ah8868b-031517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10228888
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127860.12499892461428846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG28600.026.84389538
ATAGGGG22750.017.49985537
GAGCGGG11400.016.9845169
AGCGGGG12150.016.29803510
GGGAGAG32900.015.3857195
GGGGAGA38850.014.8444514
TATAGGG24050.014.17628336
AGGGGGG81150.013.87991139
GTAGGGG15900.013.00373237
TGGGGGA19800.012.4428520
AGGGGGT13700.012.2038939
GGGGGAT14900.011.22009321
GGGGGTA15500.011.21146620
GTGGGGA33450.011.17899619
CGGGGGG37400.011.17602212
TGGGGAA36650.011.10362420
GGGGAAA51000.011.04177621
TTAGGGG17200.010.99783637
GAGGGGG53450.010.8263849
GGAGAGC14300.010.6180756
Produced by FastQC (version 0.11.2)