FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n02_ah8868a-1217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n02_ah8868a-1217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11707761
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC469780.4012552015709921Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122830.10491331348496097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC56350.041.18556644
GTGGTCG59650.039.0175442
TGGTCGC60150.038.54707743
AGCGTCG64050.037.6137311
GTCGTGT63600.037.5918714
CTCGGTG59700.037.43725638
GCGTCGT64450.037.17085612
GAGCGTC65700.036.6356110
CGTCGTG65350.036.62261613
CGTGTAG64800.036.59086616
ATCTCGG61750.036.2987936
GATCTCG61300.036.02731335
GGTGGTC64450.035.9409941
GTGTAGG65800.035.80078517
GATCGGA66700.035.7531552
GTAGGGA66400.035.6781719
TGTAGGG67050.035.10025418
AGAGCGT69750.034.6979339
TCGGTGG67050.034.3503139
AGATCGG70650.033.9845961
Produced by FastQC (version 0.11.2)