FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n02_ah8868a-031517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n02_ah8868a-031517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10645137
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117110.11001267527134691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG16800.020.55700538
AGGGGGG45950.012.25587739
ATAGGGG16700.010.93202337
TGGGGGA16550.09.83574720
GGGGAGA21100.09.69581522
GGGGGTA11350.08.72148120
GGGGGAT11900.08.50336121
GTAGGGG13800.08.12886737
GGGGGAG36550.08.06486521
GGGGGGA44850.08.04368420
TATAGGG20700.07.75695736
TGTGGGG22850.07.70092136
GGGAGAG19450.07.690900323
TGGGGGG57300.07.63955338
AAGGGGG21050.07.524019738
CGGGGGG22450.07.447204612
GGGGGAA22200.07.431592520
AGCGGGG8101.0913936E-117.33415910
GGGTGGG55700.07.26569317
GAGGGGG35250.07.24059969
Produced by FastQC (version 0.11.2)