FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n02_ah8583a-030316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n02_ah8583a-030316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15684937
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG695150.4431959146536578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG63200.028.68058238
ATAGGGG45250.022.84701337
GAGCGGG19700.022.0025719
TATAGGG46850.020.23593936
AGCGGGG24850.018.15101410
GGGAGAG65800.017.7938545
GGGGAGA80800.015.7447884
TGGGGGA43300.014.42761120
TGGGGAA70450.014.42530420
AGGGGGT31100.014.14667639
GTAGGGG35200.013.99766337
TGTAGGG30700.013.39858336
GGGAGCG16700.013.1760767
AGGGGGG209400.013.15262439
GGGAAAA133900.013.06034722
GGAGAGC24200.013.0028886
GTGGGGA72400.012.9122919
GTGGGCG18800.012.75432844
GGAGCGG17250.012.3726198
GGAGAGG62300.012.3269776
Produced by FastQC (version 0.11.2)