FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n02_ah8583-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n02_ah8583-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12266051
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161160.13138702912616293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG27850.026.06561538
GAGCGGG10300.019.2245169
ATAGGGG23950.017.26659837
GGGAGAG32450.015.8704585
AGCGGGG12050.015.70191510
GGGGAGA36850.014.6341764
TATAGGG30000.013.49093836
AGGGGGG85350.013.01605939
CGGGGGG29450.012.77312412
AGGGGGT15950.012.27498539
GCGGGGG30250.011.78232211
GGGGGCG39450.011.70999844
GGGGGTA17250.011.34935920
GTAGGGG15450.011.10505237
GGGGGGC44950.010.61984443
GGGAGCG8950.010.5715787
GGGGTAA21100.010.5297121
GGGGGAG66150.010.5178363
AAGGGGG36650.010.26363938
AGAGGGG26850.010.2428588
Produced by FastQC (version 0.11.2)