FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n02_ah8583-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n02_ah8583-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17407860
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG543210.3120486952445619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG141800.033.80341738
TATAGGG79250.032.0024736
ATAGGGG90350.031.55215337
GAGCGGG39350.030.5281129
AGCGGGG43700.028.3953510
GGGAGAG122950.025.076625
GGAGAGC35550.023.8302926
AGGGGGT54500.023.08848239
TGGGGGA68300.022.705820
TGGGGAA117150.021.8000920
GAGAGCG34700.021.7502447
GGGGAGA158550.021.2259964
TTATAGG53600.021.01205635
GGGGAGC28600.020.8502586
GTAGGGG54250.020.84085537
AGAGCGG37050.020.5473048
TGTAGGG46300.020.47621536
GGGAGCG26650.020.3938487
GTGGGCG32600.020.04176544
GGAGAGG98900.019.9351716
Produced by FastQC (version 0.11.2)