FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n02_ah8189b-031517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n02_ah8189b-031517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13389986
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218190.16295013303225261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG14600.020.6416338
AGGGGGG40750.010.8507439
ATAGGGG17350.09.8888837
GAGCGGG7650.08.9158469
GGGGAGA26050.08.0262554
GGGAGAG24150.08.0190035
AGCGGGG9650.07.751945510
GAGGGGG29550.07.7435169
TATAGGG21000.07.22731336
GTAGGGG14100.06.708127537
AAGGGGG24550.06.4514538
GGGGGGA46250.06.4272042
GGGGGCG26850.06.390522544
AGGGGGT11051.8189894E-126.370575439
GGGGGAG40500.06.25115163
CGGGGGG26000.06.17640212
GGGTTGG29100.06.046786317
GGGGGAA25150.06.03523121
GGGGGGC31350.06.034646543
GGGGGGT75000.06.01179314
Produced by FastQC (version 0.11.2)