FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n02_ah119c-040114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n02_ah119c-040114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11815801
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG381410.3227965670715003No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC330400.27962556241426206Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC42750.038.4918844
GTGGTCG46100.036.1242342
AGCGTCG49600.034.9531911
CTCGGTG45800.034.77503238
GCGTCGT49950.034.61651612
GTCGTGT51950.033.53208514
CGTGTAG50950.033.4138816
TGGTCGC49550.033.2538443
CGTCGTG53250.032.7576813
ATCTCGG49650.032.386336
GATCGGA53900.032.3144042
GGTGGTC52250.031.99873441
GTGTAGG53300.031.94092817
GATCTCG50100.031.61283935
GTAGGGA54050.031.5807119
GAGCGTC55150.031.51560810
AGATCGG55250.031.4553781
TCTCGGT52650.030.49875837
TGTAGGG55900.030.41466718
AGAGCGT57800.030.1091359
Produced by FastQC (version 0.11.2)