FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n02_ah119a-040114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n02_ah119a-040114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9085914
Sequences flagged as poor quality0
Sequence length50
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG297230.32713274635881434No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC202410.22277340507515261Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC26500.037.7698644
TGGTCGC27300.036.74342743
GTGGTCG27400.036.6898142
GCGTCGT30950.034.6154312
AGCGTCG31250.034.2891611
CTCGGTG28700.033.80135738
ATCTCGG29400.032.9943836
GTCGTGT32350.032.8372414
CGTCGTG32600.032.7246713
CGTGTAG32400.032.51539616
GATCGGA32700.032.3928072
GATCTCG29700.032.36557435
GAGCGTC33400.032.0160510
GGTGGTC31800.031.7517641
AGAGCGT34250.031.2859029
GTAGGGA33500.031.25455319
AGATCGG34300.031.1502631
TGTAGGG33700.030.93409518
TCGTGTA34800.030.77838715
AAGAGCG35900.029.9712128
Produced by FastQC (version 0.11.2)