FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n01_ah8868b-1217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n01_ah8868b-1217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14887585
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG445610.2993165110392317TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC266730.1791627050324146TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC51950.026.72180744
AGATCGG106200.024.4104371
CCGATGT107200.023.29303234
CAGTCAC108750.023.14279728
ACGTCTG109750.023.03169316
ACACGTC110850.023.00167814
CACGTCT111200.022.85014515
GCACACG111700.022.80687312
CGTCTGA111000.022.77217317
TCCAGTC112300.022.50878526
CACCGAT113500.022.2326432
CCAGTCA113250.022.22314327
CACACGT114750.022.21992113
AGTCACC113450.022.10647429
GTCACCG113600.022.09672430
CGATGTA113250.022.02954935
ACCGATG114600.021.80821433
TATCTCG97100.021.70593640
CTCGTAT99550.021.32600643
TCACCGA119950.021.03713831
Produced by FastQC (version 0.11.2)