FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n01_ah8868b-031517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n01_ah8868b-031517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10228888
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT982660.9606713848074199TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG123850.040.4114928
CGTGGCC123750.040.25036633
ACGTGGC124300.040.10707532
CACGTGG125150.039.97492231
GCACACG126850.039.9057111
GATCGGA123000.039.559591
ACACGTC128200.039.46813613
ACGTCTG127950.039.45870215
GTCACGT127400.039.30288729
GGCCTTA126500.039.09727536
CACGTCT129300.039.0472914
CGTCTGA129350.038.98060616
CACACGT130700.038.7805212
TATCTCG120500.038.76158541
GTGGCCT128600.038.66337234
CAGTCAC129750.038.55618727
AGCACAC131400.038.5071510
TCCAGTC129900.038.4429825
TCACGTG132150.038.04020330
GAACTCC131400.038.03743421
Produced by FastQC (version 0.11.2)