FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n01_ah8868a-1217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n01_ah8868a-1217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11707761
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG438360.37441830252599106TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC254690.2175394595089531TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG69250.035.018911
CGTATGC44300.030.09566944
GCACACG92950.026.12891612
ACGTCTG94400.025.58763916
ACTGACC93350.025.50024233
ACACGTC95150.025.43229714
GATCGGA95300.025.394762
CACACGT96100.025.18088513
CACGTCT96500.025.09919715
CGTCTGA97100.024.89868717
CAGTCAC98850.024.4148428
AGCACAC100450.024.17802611
TCCAGTC99850.024.08178326
CACTGAC99050.024.07690232
CCAGTCA100300.024.03974527
GAGCACA101850.023.95367810
AGAGCAC103050.023.6749449
CTGACCA100750.023.47421534
TCGTATG100200.023.36194444
TCGGAAG103800.023.3358254
Produced by FastQC (version 0.11.2)