FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n01_ah8868a-031517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n01_ah8868a-031517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10645137
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC530600.4984435616000057TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG68450.038.15001329
CACGGCT68650.037.91103731
TCACGGC69150.037.76417530
GCACACG70950.037.33211
AGTCACG71450.036.67135228
CGGCTAC72000.036.0866933
ACACGTC74400.035.71915413
CGTCTGA74250.035.7024316
GATCGGA72200.035.692911
ACGGCTA73050.035.59760732
ACGTCTG74600.035.59390615
CATCTCG69050.035.5575839
CACACGT74950.035.51574312
CACGTCT76000.034.9961214
GGCTACA73300.034.93578334
CGTATGC75900.034.75401744
CAGTCAC77900.033.74814227
AGCACAC79300.033.59527210
TCCAGTC78450.033.56731425
GAGCACA80400.033.2721339
Produced by FastQC (version 0.11.2)