FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n01_ah8583b-030316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n01_ah8583b-030316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13977720
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC227030.16242276995103638TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG40450.032.1960211
ACACGTC42700.030.49950613
CACACGT42850.030.3927412
ACGTCTG43650.029.43252815
CACGTCT45600.028.55984314
GATCGGA45700.027.6894131
AGCACAC48900.027.21732710
CGTCTGA47800.027.19954316
CGTATGC44450.026.92499544
CATCTCG41400.026.57097239
CAGTCAC47800.026.46412527
GAACTCC49300.026.01527221
TCCAGTC50700.025.07994725
GAGCACA53100.025.0644539
CCAGTCA51950.024.77317626
TCGGAAG55700.024.0554713
CTCGTAT47300.023.81444242
AGAGCAC55350.023.8071068
ACTCCAG54800.023.44422523
GTCTGAA56850.023.10180717
Produced by FastQC (version 0.11.2)