FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n01_ah8583a-030316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n01_ah8583a-030316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15684937
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2343411.4940512671488575TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG178460.11377795141925019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG289800.041.59192711
ACACGTC293450.041.0894613
GATCGGA285000.040.895621
CACACGT295400.040.82579412
ACGTCTG296500.040.66652315
CACGTCT297000.040.5538814
CGTATGC291750.040.53894844
CGTCTGA298250.040.34664516
AGCACAC301000.040.0586810
CAGTCAC297650.040.0453127
GAGCACA302650.039.869499
CCAGTCA299300.039.8165626
GAACTCC301200.039.76952421
TCCAGTC300900.039.6412725
AGAGCAC306400.039.475128
AGTCACC301600.039.45519628
TCGGAAG307000.039.4227333
CTCGTAT289200.039.36797742
TCGTATG299150.039.13204243
GTCACCT305050.038.99454529
Produced by FastQC (version 0.11.2)