FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n01_ah8583-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n01_ah8583-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12266051
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC915940.7467276958166895TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG118750.039.25574511
ACACGTC120100.038.887613
CACACGT120650.038.72855412
ACGTCTG121550.038.27890815
GATCGGA118550.038.260651
CACGTCT121950.038.1894314
CGATCTC107650.037.99218438
CGTCTGA123050.037.77652416
GATCTCG109100.037.5882339
CACGATC109000.037.5821936
CGTATGC122750.037.5482544
AGCACAC126200.037.06037510
CAGTCAC124550.037.05798727
CATCACG123500.036.96379533
TCCAGTC124650.036.8864625
GAGCACA127850.036.7198879
GAACTCC125700.036.71775421
CCAGTCA127200.036.19887526
AGAGCAC130350.036.1509448
ATCACGA121100.035.95203434
Produced by FastQC (version 0.11.2)