FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n01_ah8583-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n01_ah8583-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17407860
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC5191672.9823711817535297TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC514190.2953780648511649TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG716550.043.0034311
ACACGTC721550.042.7481213
CACACGT722600.042.6860112
ACGTCTG721900.042.59026315
CACGTCT722750.042.54321714
AGCACAC726800.042.46984510
CGTATGC695200.042.4275144
GATCGGA699900.042.4152151
CGTCTGA728100.042.24572416
GTCACAC721500.042.17357629
GAGCACA732350.042.1658979
TCGGAAG735250.042.111293
CACACAG726450.041.95271331
GAACTCC730450.041.90224521
AGAGCAC738600.041.8808148
GATCTCG582950.041.80423739
CAGTCAC729400.041.75299527
TCCAGTC730850.041.6543925
GTCTGAA738600.041.6121517
AGTCACA733850.041.53878428
Produced by FastQC (version 0.11.2)