FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n01_ah8189b-031517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n01_ah8189b-031517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13389986
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC326630.24393602801377087TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG55250.034.4821911
ACGTCTG58450.032.85783415
ACACGTC59100.032.53380213
CACGTCT59800.032.22654714
GATCGGA57600.032.0164761
CACACGT59900.031.9889912
CACTAGC61650.031.29587631
CGTCTGA61500.031.228316
CGTATGC56100.030.58945744
CAGTCAC63800.030.20629927
AGCACAC63650.030.17334410
GAACTCC64800.029.73937621
TATCTCG56500.029.47772639
CCAGTCA67200.028.74283426
ACTAGCT67100.028.59013232
GAGCACA67700.028.4655639
TCCAGTC68150.028.14806225
AGAGCAC69950.027.5816028
TCACTAG69700.027.55530
GCTTATC67900.027.15257636
Produced by FastQC (version 0.11.2)