FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n01_ah119c-040114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n01_ah119c-040114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11815801
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT466770.39503881285746095TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA298670.2527716910601321TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG54750.032.356791
CTCGTAT73650.030.91692444
CGTCCGC99300.023.7068733
ACGTCCG100700.023.35493932
GCACACG103500.023.27450211
AGTCACG103600.023.04046628
CACGTCC103800.022.76331931
ACACGTC107400.022.55214313
CCGCACA98850.022.52408636
GTCACGT106850.022.38093229
GATCGGA106650.022.3677371
CACACGT108600.022.30294812
CGCACAT98200.022.24711837
GTCCGCA105550.022.17803634
TCACGTC106800.022.14440530
CATCTCG96600.022.11473841
ACGTCTG109150.022.04938515
CACGTCT110350.021.92931714
TCCGCAC106350.021.84589235
CGTCTGA111750.021.67418316
Produced by FastQC (version 0.11.2)