FastQCFastQC Report
Fri 12 Jan 2018
HVHMTAFXX_n01_ah119a-040114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMTAFXX_n01_ah119a-040114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9085914
Sequences flagged as poor quality0
Sequence length50
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT363010.3995305260428395TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA158430.1743688086856204TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT60700.033.96057544
AGATCGG34100.031.7520051
CGTCCCG76000.028.3985635
GTCCCGA70500.028.0555436
CCCGATC64050.028.02927838
CCCGTCC78100.027.66252133
GCACACG81850.027.19972411
ACCCGTC79200.027.19468732
GATCTCG65250.027.17661741
CCGTCCC79500.027.1477134
CGATCTC65500.027.10632940
TCACCCG79650.026.98535530
CCGATCT65650.026.94400839
ACACGTC83650.026.61443113
TCCCGAT70100.026.61437837
CACCCGT81050.026.57366431
CACACGT83800.026.5142912
ACGTCTG84150.026.45600515
CGTCTGA84200.026.25755116
GATCGGA83950.026.24061
Produced by FastQC (version 0.11.2)