FastQCFastQC Report
Wed 26 Oct 2022
HVHMMBGXM_n01_T14_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMMBGXM_n01_T14_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7667592
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1572442.0507611776943793No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG1554532.0274031273442823No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1199161.5639329792195515No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG1027381.3398991495635135No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG959251.2510446565231952No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG920561.2005855293291556No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG852771.1121744610302686No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG849311.1076619621909982No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG697460.9096206475253247No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC695360.9068818476517791No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC602480.7857486418161008No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG578010.7538351023372135No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC494470.6448830349867337No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG489230.6380490772070293No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG483520.630602149931817No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG455080.5935109745015124No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG422540.5510726183657138No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG418990.5464427423890056No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG395730.5161072733134471No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC380960.49684438086950894No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG367390.4791465169247399No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG365190.47627729800959673No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC356770.46529601470709453No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC352730.46002708542655896No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG344150.44883713165750083No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG339130.44229009576931066No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG333070.4343867018485073No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC322500.42060140915166067No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG319970.4173018073992461No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG319880.4171844302618084No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG295170.38495788508308737No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG277190.3615085414038723No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG268040.3495751990977089No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG264540.3450105326417994No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG261010.3404067404734107No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG247750.32311317555759356No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC239420.31224926939252895No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG235150.3066803763163194No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG225600.29422535784376636No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG224280.29250382649468043No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG216510.2823702669625614No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG213030.2778316843149714No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG210600.27466250160415423No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG208450.2718584922098098No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG205240.2676720409745328No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG202820.26451590016787535No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG200290.2612162984154608No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG199630.2603555327409179No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG197880.25807319951296315No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG196230.2559212853266058No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC192200.25066539795023No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTG184800.24101438887202137No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG183550.23938415085205367No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG181590.23682793763674437No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG180230.23505423867101952No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG171900.22419033250595496No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC170010.2217254126197638No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAG169720.22134719739913133No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACAG168080.21920832511693372No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACAG167560.21853014610062715No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC166180.21673036332658285No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACAG160410.20920518462641205No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAG160160.2088791370224185No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTC160080.20877480178914057No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC159250.20769232374388205No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC157650.20560561907832345No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG149860.19544597573788483No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAAACCCTACCAACTG146300.1908030578570169No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG145750.19008575312823112No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG144810.18885981413721545No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACAG143620.1873078275422062No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAACCCTACCAACTG142230.18549500286400214No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC140280.18295183155285258No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG139220.18156938971192No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC137930.17988698407531334No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG137680.17956093647131982No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG134060.17483976716549343No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACAG133840.1745528452739791No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG133110.173600786270318No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC130720.1704837711761398No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACAG128530.1676275941651564No Hit
GCTAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG128270.16728850465700312No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAAACCCTACCAACTG127620.16644078088661995No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC126740.16529309332056272No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG126370.16481054286665225No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG126260.1646670819208951No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTC124330.16214999441806502No Hit
GCTAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTG121650.15865476410325433No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC118720.1548334861844501No Hit
GCTAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACAG113640.14820819887130146No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTG112260.14640841609725713No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACAG112190.14631712276813894No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAACCCTACCAACTG111230.14506509996880376No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACAG110440.1440347895401842No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAG110230.14376090955282964No Hit
GATAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTC109420.14270451531589057No Hit
GATAGCTCCGTTCAACTGGAAAGGACGAAACACCGAAAACCTACCAACAG109330.1425871381784529No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG108640.14168724679143074No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC106550.1389614888220448No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG106130.13841372884733563No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG105210.1372138736646394No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG104110.13577926420706787No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACAG103940.1355575518363523No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG102480.13365343382903003No Hit
GCTAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG102270.13337955384167546No Hit
GCTAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTC101320.13214057294650003No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC100540.13112330442204018No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC100400.1309407177638038No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACAG100380.1309146339554843No Hit
GATCGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG98220.12809758265698018No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC96880.12634996749957483No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG96650.1260500037039008No Hit
GGGTCGAGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96350.12565874657910853No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACAG95570.1246414780546487No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC94780.12361116762602913No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAAAACCTACCAACTG94050.12265910862236801No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG94040.12264606671820828No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACAG93570.12203309722270043No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAACCCCTACCAACTG93150.12148533724799129No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTG92990.12127666678143542No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACAACGAAAACCTACCAACAG92260.1203246077777743No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG91470.11929429734915474No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACAACGAAAACCTACCAACTG90600.11815965168725723No Hit
GATAGCTCGGTTCATGTGGAAAGGACGAAACACCGAAACCCTACCAACTG89870.1172075926835961No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG88540.1154730194303505No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACAG88360.11523826515547515No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACAG87900.11463833756412704No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACAACGAAACCCTACCAACTG87360.113934074739501No Hit
GCTAGCTCCGTTCATGTGGAAAGGACGAAACACCGAAACCCTACCAACTG87190.1137123623687854No Hit
GCTAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG86350.11261684241936712No Hit
GATAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTC86340.11260380051520738No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG85330.1112865681950735No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG85270.11120831677011506No Hit
GCTAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACAG85170.11107789772851764No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTG84550.11026929967061366No Hit
GATAGCTCCGTTCAAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG83470.1088607740213616No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAACCCCTACCAACTG82660.10780437978442255No Hit
GCTAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG82290.1073218293305121No Hit
GATAGCTCCGTTCAACTGGAAAGGACGAAACACCGAAAACCTACCAACTG81650.10648714746428867No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACAG79570.10377443139906245No Hit
GCTAGCTCCGTTCAACTGGAAAGGACGAAACACCGAAAACCTACCAACAG79300.10342229998674944No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTC77940.10164860102102459No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACAG77780.10143993055446872No Hit
GATAGCTCCGTTCAACTGGAAAGGACGAAACACCGAAACCCTACCAACTG77380.10091825438807908No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACAG77320.10084000296312064No Hit
GCTAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTC77090.10054003916744657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)