FastQCFastQC Report
Wed 26 Oct 2022
HVHMMBGXM_n01_T0_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMMBGXM_n01_T0_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9526556
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGGT5590265.868080762869603No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT5495425.768527472047611No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT3221203.3812849050590787No Hit
TAAGTAGACTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGGT3192863.3515364839087702No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT1845831.9375627456554079No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGGT1838351.9297110099389538No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT1694921.7791529278786584No Hit
TAAGTAGACTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1492111.5662638208393462No Hit
TAAGTAGACTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT1434141.5054128690368271No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGCT1310771.3759117145797495No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCAATACCAACTGGT1289171.353238253152556No Hit
TAAGTAGACTCTTGTGGAAAGGACGAAACACCGAACCAATACCAACTGGT1176001.2344440110360975No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCAATACCAACTGCT1092921.1472351603244657No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGGT1089341.143477244032366No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT1040341.0920420769058619No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGGT844380.8863433962913775No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGGT842240.8840970440944241No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT824230.8651919959322132No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT821800.8626412315216538No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCACTACCAACTGCT806810.8469062691700967No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGGT798300.8379733452466978No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT750300.7875878754084896No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGGT725860.7619332736825355No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT721060.7568947266987146No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT704920.7399526124656172No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT696950.731586525077898No Hit
TAAGTAGACTCTTGTGGAAAGGACGAAACACCGAACCAATACCAACTGCT673360.7068241660469954No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCACTACCAACTGGT654970.6875202329152319No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCT621520.6524078586217307No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCACTACCAACTGCT610580.6409241702877724No Hit
TAAGTAGACTCTTGTCGAAAGGACGAAACACCGAACCACTACCAACTGGT607500.6376911026398208No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCACTACCAACTGCT600910.6307735975099501No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGGT570270.5986108725965605No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT523730.5497579607992648No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGCT516650.5423261039981291No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGGT515930.5415703219505559No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCAATACCAACTGCT502370.5273364267212621No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGCT480650.504537001619473No Hit
TAAGTAGACTCTTGTCGAAAGGACGAAACACCGAACCACTACCAACTGCT479010.5028154980666676No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCT476870.500569145869714No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCAATACCAACTGGT472440.4959189868825628No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGGT470020.49337871944488654No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCAATACCAACTGGT450440.4728256465400508No Hit
TAAGTAGAGTCTTGACGAAAGGACGAAACACCGAACCACTACCAACTGCT445220.46734622669514564No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT443330.46536229882026625No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT431140.452566488875938No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTGGT430390.451779215909716No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCACTACCAACTGGT414070.43464815616472524No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCAATACCAACTGCT412130.43261174342543096No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCAATACCAACTGCT404610.42471801981744506No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGCT383330.40238046152250617No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGGT374510.3931221314397354No Hit
TAAGTAGACTCTTGACGAAAGGACGAAACACCGAACCACTACCAACTGCT368930.3872648205710437No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCATACCAACTGGT358890.3767258597965519No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT354900.37253756761625084No Hit
TAAGTAGACTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGGT349810.3671946084188242No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCAATACCAACTGCT346080.3632792375334801No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCACTACCAACTGGT345040.36218755235365224No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGCT344600.361725685546802No Hit
TAAGTAGACTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT343170.36022461842453873No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCACTACCAACTGGT333440.35001106380941865No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGGT331180.3476387479378697No Hit
TAAGTAGACTCTTGACGAAAGGACGAAACACCGAACCAATACCAACTGCT326780.3430200798693673No Hit
TAAGTAGACTCTTGACGAAAGGACGAAACACCGAACCACTACCAACTGGT324300.3404168305943932No Hit
TAAGTAGAGTCTTGACGAAAGGACGAAACACCGAACCAATACCAACTGCT323410.3394826000078097No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCAATACCAACTGGT311420.3268967295211407No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTGCT310440.3258680261786106No Hit
TAAGTAGACTCTTGTCGAAAGGACGAAACACCGAACCAATACCAACTGGT308490.3238211164664334No Hit
TAAGTAGACTCTTGTGGAAAGGACGAAACACCGAACCCATACCAACTGGT300830.315780435238086No Hit
TAAGTAGACTCTTGTCGAAAGGACGAAACACCGAACCAATACCAACTGCT300660.31560198669907574No Hit
TAAGTAGAGTCTTGACGAAAGGACGAAACACCGAACCACTACCAACTGGT298570.31340811936653706No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT296460.3111932580882325No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTGGT279910.2938207679669337No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCT268730.2820851522837844No Hit
TAAGTAGACTCTTCTCGAAAGGACGAAACACCGAACCAATACCAACTGGT250060.26248730391129804No Hit
TAAGTAGACTCTTGACGAAAGGACGAAACACCGAACCAATACCAACTGGT243360.25545433207971485No Hit
TAAGTAGAGTCTTGACGAAAGGACGAAACACCGAACCAATACCAACTGGT231280.24277398883709916No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTGGT222490.23354714967297732No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT218560.229421839329974No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGGT215360.2260628080074268No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCATACCAACTGCT193320.20292747977338296No Hit
TAATTAGAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT190490.19995683644750528No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCA182350.19141230052077582No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTGCT176480.18525057743847828No Hit
TAAGTAGACTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGGT168870.17726238107454573No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGCT165470.17369341029433932No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGCT148120.1554811623424037No Hit
TAAGTAGACTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT146600.15388562246419377No Hit
TAAGTAGAGTCTTCTGGAAAGGACGAAACACCGAACCCATACCAACTGGT139660.14660072328341953No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT139130.14604438372062264No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT137780.14462729238142305No Hit
TAAGTAGAGTCTTCACGAAAGGACGAAACACCTAACCAATACCAACTGCT136750.14354610417447816No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCCCTACCAACTGGT135520.1422549765098741No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCA133630.14027104863499465No Hit
TAAGTATAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGCT132160.13872799362119953No Hit
TAAGTAGAGTCTTCAGCAAAGGACGAAACACCGAACCAATACCAACTGCT131530.13806668432957303No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGCA129620.13606176250892768No Hit
TAAGTAGACTCTTCACGAAAGGACGAAACACCGAACCAATACCAACTGCA128370.13474964089855768No Hit
TAAGTAGAGTCTTCTCGAAAGGACGAAACACCGAACCCCTACCAACTGCT124040.130204451640236No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGGT116560.1223527159237819No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCAATACCAACTGGT113700.11935058167925534No Hit
TAAGTAGACTCTTCACCAAAGGACGAAACACCTAACCAATACCAACTGCT109930.11539322290237941No Hit
TAAGTAGACTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTGCT108820.11422805891237084No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCACTACCAACTGCT105740.11099499126441915No Hit
TAAGTAGAGTCTTCAGGAAAGGACGAAACACCGAACCAATACCAACTGCA103940.10910553614548638No Hit
TAAGTAGAGTCTTCACCAAAGGACGAAACACCGAACCACTACCAACTGCT98210.10309077068355028No Hit
TAATTAGAGTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGGT98100.10297530398183771No Hit
TAAGTAGACTCTTCTCCAAAGGACGAAACACCGAACCAATACCAACTGCT96340.10112783675443676No Hit
TAAGTAGACTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTGCT95730.10048752140857621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)