FastQCFastQC Report
Wed 2 Nov 2022
HVHMMBGXM_n01_R21_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMMBGXM_n01_R21_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7435215
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG2137052.8742275778171846No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG1975802.657354225802482No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1753372.358196770369115No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG1397851.880039783651179No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1376251.8509888416138605No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1334761.7951868237838449No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG877991.1808535462659788No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC835401.1235720823136923No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG775391.0428615715887166No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC715970.9629445819656862No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG657260.8839825075670308No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC654300.8800014525471019No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG641960.8634047569572635No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG573140.7708452277439186No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG572910.770535889009262No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG553740.7447531779511419No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG518610.6975050486098923No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG515870.6938198828144176No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC471320.6339023148624485No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG470100.6322614746177481No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC428330.5760828705020635No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG407010.5474085147504141No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG403110.5421632057714538No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG402370.5411679420164717No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG365500.49157959789999345No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG341080.45873589398558073No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG324210.43604657027402705No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG319620.42987324509109687No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG295000.39676055097263496No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG290040.3900895939122137No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC284110.3821140343621536No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG279340.37569861799557913No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG275000.36986153056771054No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG265770.35744763265083795No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG250260.33658744232681903No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG248790.33461036432705715No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC247170.33243154367425826No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG241140.3243214890221735No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG240950.32406594832832675No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC240170.3230168865325347No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC236480.3180540172678261No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAG227350.30577461445297816No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAG221060.29731487253562944No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG214080.2879271144143108No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG206610.2778803302930716No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG204400.27490798853832743No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG201600.271142125681638No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG199120.2678066471514274No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACAG191750.25789435813221273No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTG190700.25648215956095416No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACAG183470.246758163684574No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG176870.23788148695094896No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACAG173720.23364489123717339No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTC171860.23114328233951542No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG169220.22759261164606537No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC168500.2266242469114881No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG167110.22475476499334585No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAACCCTACCAACTG165920.22315427327925286No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG161910.2177610196880655No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAAACCCTACCAACTG160510.2158780882597208No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACAG156860.2109690170358221No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG156720.21078072389298766No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAAACCCTACCAACTG156300.21021584446448421No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC156160.21002755132164974No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG153270.20614064287313816No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC153030.20581785462827906No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACAG152940.20569680903645693No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC146070.19645699552736537No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG142600.191790015487111No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG136330.18335717259016718No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG134740.1812187004679757No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG134410.18077486663129447No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG134280.18060002299866246No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTC133190.17913402638659406No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACAG132860.1786901925499128No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC129910.17472258704018645No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG127990.1721402810813137No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG127680.17172334626503738No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACAG126000.16946382855102374No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACAG124830.16789023585733567No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC123610.16624939561263527No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG122540.1648102980209718No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG120840.16252388128655323No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG118940.15996847434808542No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG118640.15956498904201158No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTG118540.15943049393998696No Hit
GATCGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG118470.1593363473685697No Hit
GATAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTC117650.1582334875319678No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG117520.1580586438993358No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC114390.15384894720596512No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC110980.1492626642269255No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAAAACCTACCAACTG110250.14828084998214577No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG109370.1470972930843291No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC108060.14533540724780655No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACAACGAAACCCTACCAACTG107490.1445687851662662No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTG107290.14429979496221695No Hit
GATAGCTCGGTTCATGTGGAAAGGACGAAACACCGAAACCCTACCAACTG105730.14220167137063286No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACAG103800.13960591590155766No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACAG103720.13949831981993796No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG103440.13912173353426902No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAACCCCTACCAACTG103250.13886619284042223No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC102400.13772298447321296No Hit
GATAGCTCCGTTCAACTGGAAAGGACGAAACACCGAAAACCTACCAACAG99550.13388987406551123No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACAACGAAAACCTACCAACTG98100.13193969508615422No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC98090.13192624557595176No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACAG97890.13165725537190248No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG96040.12916909598444698No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACAG95920.12900770186201743No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTG95830.12888665627019527No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAACCCCTACCAACTG94170.12665403757658655No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAAAACCTACCAACTG92600.1245424644748No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACAG92530.12444831790338275No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACAACGAAAACCTACCAACAG91330.1228343766790873No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC91250.12272678059746758No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG91110.12253848745463311No Hit
GATAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTC90440.12163737027106815No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTC88340.11881297312855109No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC87920.11824809370004769No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG87660.11789840643478366No Hit
GGGTCGAGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86430.1162441166798808No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACAG85680.11523540341469615No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTC84680.11389045239444992No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG83580.11241100627217909No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAACACCTACCAACTG82700.11122744937436241No Hit
GATAGCGCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC81940.11020528659897529No Hit
GATAGCTCGGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTG81510.10962695766026942No Hit
GATAGCTCCGTTCAAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG80460.10821475908901089No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACAACGAAAACCTACCAACAG79180.1064932217830957No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG78600.1057131501913529No Hit
GATCGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG78360.10539036194649383No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTC76380.10272735892640629No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACAACGAAAACCTACCAACTG76370.10271390941620384No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACAACGAACCCCTACCAACTG75950.10214902998770042No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTG75310.10128826133474285No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC75240.1011941147633256No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG75080.10097892260008622No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACAG74730.10050818974300005No Hit
GCTAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG74670.10042749268178526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)