FastQCFastQC Report
Wed 2 Nov 2022
HVHMMBGXM_n01_R20_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMMBGXM_n01_R20_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7596060
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1557272.050102289871328No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG1540102.027498466310166No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1187651.56350792384473No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG1017161.3390626193052713No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG952351.253742071547618No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG913131.2021100412582313No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG847091.1151702329892075No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG843561.1105230869687706No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG690610.9091687006158455No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC689990.9083524880003582No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC597840.7870395968436268No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG572930.7542462803084757No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC490390.6455846846918007No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG484170.6373962290977164No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG479890.6317617291069317No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG450560.593149606506531No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG418990.5515885867146916No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG414830.5461120633591625No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG393030.5174129746210535No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC378040.49767905993370243No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG363610.4786823695442111No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG362170.47678664992114334No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC354220.4663206978354568No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC350050.4608310097603231No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG341470.44953568033954444No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG335660.4418869782492503No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG329780.43414612312172357No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC320150.421468498142458No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG317730.41828263599813587No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG317030.41736110562581125No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG292020.3844361418946138No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG274750.36170067113740545No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG265040.3489177284013028No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG262360.34538958354726No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG259060.3410452260777298No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG246130.3240232436289339No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC237720.3129517144414341No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG232780.30644834295674334No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG223270.29392869461273347No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG222030.29229626938175846No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG214730.2826860240703733No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG210990.277762418938239No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG208600.2746160509527308No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG206610.27199627175140795No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG203880.268402303299342No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG201470.26522960587462446No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG198710.26159614326374464No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG197660.2602138477052577No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG196480.25866041079191054No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG194660.25626443182386655No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC191000.2514461444485694No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTG182880.24075639212960404No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG182020.23962422624360524No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG179830.2367411526501897No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG178990.2356353162034002No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG170230.22410302182973804No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC169020.22251009075757697No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAG168080.2212726071147411No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACAG166360.2190082753427435No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACAG166080.21863966319381364No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC165060.21729686179414062No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTC159090.20943752419017228No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACAG158860.20913473563926563No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAG158760.20900308844321924No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC158030.20804206391208074No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC156420.20592254405573415No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG148610.19564089804451254No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAAACCCTACCAACTG144900.19075678707119215No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG144500.19023019828700669No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG143690.18916385599903107No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACAG142410.18747877188963752No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAACCCTACCAACTG140680.1852012753980353No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC138990.18297643778485162No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG137680.18125185951664416No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC136930.18026450554629636No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG136290.17942196349159958No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG133120.1752487473769296No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACAG132420.17432721700460502No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG132080.17387961653804734No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC129830.17091755462700398No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACAG127550.16791599855714673No Hit
GCTAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG127330.1676263747258447No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAAACCCTACCAACTG126490.1665205382790552No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC125480.16519090159898686No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG125120.16471697169321992No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG125110.16470380697361525No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTC123360.16239998104280376No Hit
GCTAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTG120670.1588586714691564No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC117690.15493558502697452No Hit
GCTAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACAG112780.1484717077010977No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTG111130.14629952896633253No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACAG110980.146102058172263No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAACCCTACCAACTG110320.14523318667835694No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAG109520.14418000910998596No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACAG109480.14412735023156742No Hit
GATAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTC108630.14300834906517326No Hit
GATAGCTCCGTTCAACTGGAAAGGACGAAACACCGAAAACCTACCAACAG108050.14224479532810433No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG107520.14154706518905855No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC105890.13940121589350268No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG105320.13865082687603836No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG104230.1372158724391329No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACAG103010.1356097766473672No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG102900.13546496473171618No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG101590.13374038646350872No Hit
GCTAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG101520.13364823342627627No Hit
GCTAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTC100510.13231859674620788No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACAG99480.13096263062693028No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC99430.1308968070289071No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC99390.13084414815048856No Hit
GATCGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG97330.12813221591193327No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC96210.12665776731621395No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG95720.1260126960555867No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACAG94900.12493318904800647No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC94100.1238800114796355No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAAAACCTACCAACTG93330.12286632807007843No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG93280.12280050447205525No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACAG92850.12223442152905586No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAACCCCTACCAACTG92300.12151036195080082No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTG92070.12120757339989417No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACAACGAAAACCTACCAACAG91330.120233384149151No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG90940.11971996008457017No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACAACGAAAACCTACCAACTG89810.11823234676924617No Hit
GATAGCTCGGTTCATGTGGAAAGGACGAAACACCGAAACCCTACCAACTG89030.11720549864008448No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG87930.11575737948357437No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACAG87470.11515180238176106No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACAG87080.11463837831718023No Hit
GGGTCGAGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86680.11411178953299474No Hit
GCTAGCTCCGTTCATGTGGAAAGGACGAAACACCGAAACCCTACCAACTG86580.11398014233694838No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACAACGAAACCCTACCAACTG86480.11384849514090199No Hit
GCTAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG85620.11271632925490319No Hit
GATAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTC85560.11263734093727537No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG84810.1116499869669276No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG84650.1114393514532534No Hit
GCTAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACAG84460.11118922178076529No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTG83560.11000439701634795No Hit
GATAGCTCCGTTCAAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG82680.10884590169113989No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAACCCCTACCAACTG81820.1077137358051411No Hit
GCTAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG81730.10759525332869936No Hit
GATAGCTCCGTTCAACTGGAAAGGACGAAACACCGAAAACCTACCAACTG80800.10637093440546809No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACAG78840.10379064936295922No Hit
GCTAGCTCCGTTCAACTGGAAAGGACGAAACACCGAAAACCTACCAACAG78470.10330355473758765No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTC77250.10169745894582191No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACAG77080.10147365871254307No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACAG76670.10093390520875295No Hit
GCTAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTC76590.10082858745191585No Hit
GATAGCTCCGTTCAACTGGAAAGGACGAAACACCGAAACCCTACCAACTG76490.10069694025586949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)