FastQCFastQC Report
Wed 2 Nov 2022
HVHMMBGXM_n01_R19_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMMBGXM_n01_R19_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7369033
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG2073912.8143584103911596No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG1925312.6127037292410007No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1665612.2602829977827485No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1343031.8225322101285202No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG1333401.8094640097282777No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1247371.6927187054258002No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG852731.1571803247454584No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC806851.094919781197886No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG733080.9948116666053741No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC694360.9422674589732465No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG633790.860072142437142No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG629420.8541419206563465No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG628850.8533684134675472No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC621180.84295999217265No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG620000.8413586965888197No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG538350.7305571843686953No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG503480.6832375428363531No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG479090.6501395773366736No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC452270.6137440285584282No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG448820.6090622745209582No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC427240.5797775637590441No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG399630.5423099611577258No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG391210.5308837672459874No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG386870.5249942563698656No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG351990.4776610445359656No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG332120.4506968553404497No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG315160.4276816238982781No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG310890.4218871051330616No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG310380.4211950197536095No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG306260.41560405551176116No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC287750.3904854273281176No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG287370.3899697558689179No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG275670.37409250304619346No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC267460.3629512854671705No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG265760.3606443342023302No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC260740.35383204282027236No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG251360.341103099958977No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC244550.33186172459805785No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG239410.3248865895973054No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG234810.31864425088067866No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG233580.3169751037890589No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG231590.3142746137790399No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAG216820.29423127837804497No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAG215790.2928335373175829No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG204760.27786549469923666No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG196500.2666564256124243No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG195670.26553009058311994No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG195000.2646208803787417No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG194340.26372524047592133No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACAG188990.2564651291424533No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTG179030.24294910879080067No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG178810.24265056215652717No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACAG177310.24061501692284457No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG171490.23271710141615598No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTC165800.22499559982971984No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACAG165150.22411353022845737No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC164210.2228379218820163No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAACCCTACCAACTG163500.2218744304714065No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC160250.2174640824650941No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAAACCCTACCAACTG158080.21451932702703327No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG158030.21445147551924382No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG157950.21434291310678077No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAAACCCTACCAACTG156240.21202239154038258No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACAG154300.20938975303815302No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC152340.20672997393280773No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG151460.2055357873957139No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACAG149120.20236033683116902No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACAG146490.1987913475214455No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG146090.19824853545913013No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC142710.19366177353256525No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACTG142040.19275256332818702No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG140400.190527033872694No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG138060.18735158330814913No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG132550.17987434714975492No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG132530.17984720654663916No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG132440.1797250738326182No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACAG129920.17630535784003137No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTG127980.17367271933780185No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTC127130.1725192437053817No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC126500.17166431470723498No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC125250.16996802701249947No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACAG121470.16483845302361924No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC120320.16327786834446256No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC119300.16189369758555838No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACAG119300.16189369758555838No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTG117860.15993957416122306No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC117610.15960031662227595No Hit
GATAGCTCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG117440.15936962149579192No Hit
GATCGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG116220.15771404470573006No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTC115750.1570762405325095No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTG114710.15566492917048952No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACAG112650.1528694470495654No Hit
GATAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTC110950.15056249578472508No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTG110780.15033180065824103No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC109910.1491511844227051No Hit
GCTAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTG106450.1444558600836772No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAAAACCTACCAACTG105530.14320739234035185No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACAACGAAACCCTACCAACTG103870.14095472228174308No Hit
GATAGCTCCGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTG103720.14075116775837482No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG102550.13916344247610235No Hit
GATAGCTCGGTTCATGTGGAAAGGACGAAACACCGAAACCCTACCAACTG102240.13874276312780795No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACAG100390.13623225733959937No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAACCCCTACCAACTG99840.13548589075391573No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAACCCTACCAACAG99750.13536375803989478No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACTG98230.1333010722030964No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACAACGAAAACCTACCAACTG97030.13167263601615029No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACTG95120.12908070841859443No Hit
GATAGCTCCGTTCAACTGGAAAGGACGAAACACCGAAAACCTACCAACAG94030.12760154554878503No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAAACCTACCAACAG93620.1270451631849118No Hit
GATAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTG93240.12652949172571218No Hit
GATAGCTCGGTTCTTCTGGAAAGGACGAAACACCGAAAACCTACCAACAG91340.12395113442971419No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACACCGAACCCCTACCAACTG90700.1230826351300096No Hit
GATAGCTCCGTTCTTGAGGAAAGGACGAAACACCGAAAACCTACCAACTG90640.1230012133206623No Hit
GATAGCTCGGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACAG89070.12087067597607447No Hit
GCTAGCTCCGTTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTG88730.1204092857231064No Hit
GATAGCTCCGTTCTACTGGAAAGGACGAAACAACGAAAACCTACCAACAG88700.12036857481843276No Hit
GATAGCGCGGTTCTTCTGGAAAGGACGAAACACCGAACACCTACCAACTC87550.11880799013927609No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTC87430.11864514652058147No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTC84610.11481832148125812No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTC84210.11427550941894277No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAG83820.11374626765818527No Hit
GATAGCTCGGTTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACAG83160.11285062775536492No Hit
GCTCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC82770.11232138599460743No Hit
GCTAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAAACCCTACCAACTG81710.1108829340294717No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTC80910.10979730990484098No Hit
GCTAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAACCCTACCAACTG79630.1080603113054318No Hit
GATAGCTCGGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTG79240.10753106954467431No Hit
GATAGCTCCGTTCAAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG79230.10751749924311643No Hit
GATAGCTCCGTTCTTGTGGAAAGGACGAAACAACGAACACCTACCAACTG78800.10693397627612741No Hit
GATAGCGCCGTTCTTGTGGAAAGGACGAAACACCGAAAACCTACCAACTC78260.10620117999200165No Hit
GGGTCGAGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78070.10594334426240186No Hit
GATCGCGCGGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACTG76740.10413849415520328No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACAACGAAAACCTACCAACAG76340.10359568209288789No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACAACTACCAACTG76210.10341926817263541No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACAG76070.10322928395082502No Hit
GCTAGCTCCGTTCTACTGGAAAGGACGAAACACCGAAAACCTACCAACTG75820.10289002641187793No Hit
GATAGCGCGGTTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTG74980.10175012108101565No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAAACCCTACCAACTG74020.10044737213145878No Hit
GATAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAAACCTACCAACTC73870.10024381760809051No Hit
GCTAGCTCCGTTCTAGTGGAAAGGACGAAACACCGAAACCCTACCAACAG73800.10014882549718532No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)