FastQCFastQC Report
Wed 2 Nov 2022
HVHMMBGXM_n01_R08rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHMMBGXM_n01_R08rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTGG37.317073170731707No Hit
ATACACTATCTCTACTGGAAAGGACGAAACACAGAACACCTACCAACTCG24.878048780487805No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGAACAACTATCAACTGC12.4390243902439024No Hit
ATACACGATCTCTACTGGAAAGGACGAAACACCGAACACCTACCAACTGG12.4390243902439024No Hit
ATACACGATCTCTTCTGGAAAGGACGAAACACAGAACACCTACCAACTGG12.4390243902439024No Hit
CTAAACAAACTCTACTGGAAAGGACGAAACACAGGACAACTACCAACTCC12.4390243902439024No Hit
ATACACGATCTCTAGACGAAAGGACGAAACACCGAACAACTACCAACTGG12.4390243902439024No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACAGAACAACTACCAACTCC12.4390243902439024No Hit
CTACACTATCTCTTCAGGAAAGGACGAAACACAGAACAACTACCAACTCC12.4390243902439024No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACAGAACAACTACCAACTCG12.4390243902439024No Hit
ATACACTATCTCTAGACGAAAGGACGAAAAACAGAACAACTACCAACTCC12.4390243902439024No Hit
ATACACGATCTCTTCACGAAAGGACGAAACACAGAACACCTACCAACTGG12.4390243902439024No Hit
ATACACGATCTCTAGTGGAAAGGACGAAACACCGAACACCTACCAACTCG12.4390243902439024No Hit
ATACACTATCTCTTGTGGAAAGGACGAAACACCGAACCACTACCAACTGG12.4390243902439024No Hit
ATACACGATCTCTTGAGGAAAGGACGAAACACAGAACACCTACCAACTCG12.4390243902439024No Hit
ATACACGATCTCTAGTGGAAAGGACGAAACACAGAACAACTACCAACTCG12.4390243902439024No Hit
ATACACTAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCG12.4390243902439024No Hit
ATACACGAACTCTTGTGGAAAGGACGAAACACAGAACAACTACCAACTCC12.4390243902439024No Hit
ATAAACGATCTCTTGAGGAAAGGACGAAACACAGAACCACTACCAACTCC12.4390243902439024No Hit
ATACACTATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTGG12.4390243902439024No Hit
ATACACGATCTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACTCG12.4390243902439024No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTCC12.4390243902439024No Hit
ATACACTATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC12.4390243902439024No Hit
ATACACTATCTCTAGACGAAAGGACGAAACACAGAACAACTACCAACTGG12.4390243902439024No Hit
ATACACTATCTCTAGACGAAAGGACGAAACACCGAACACCTACCAACTGG12.4390243902439024No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTGG12.4390243902439024No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACAGAACACCTACCAACTCG12.4390243902439024No Hit
ATACACTATCTCTTGTGGAAAGGACGAAACACCGAACACCTACCAACTGG12.4390243902439024No Hit
ATACACGATCTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTGG12.4390243902439024No Hit
ATACACGATCTCTAGAGGAAAGGACGAAACACAGAACAACTACCAACTCG12.4390243902439024No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACAACTACCAACTGG12.4390243902439024No Hit
GCTAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACACTTACCAACTC12.4390243902439024No Hit
ATACACGATCTCTAGTGGAAAGGACGAAACACAGAACACCTACCAACTCC12.4390243902439024No Hit
CTAAACAAACTCTACAGGAAAGGACGAAACACAGAACAACTACCAACTCG12.4390243902439024No Hit
ATACACGAACTCTTGTGGAAAGGACGAAACACAGAACCCCTACCAACTGG12.4390243902439024No Hit
ATACACGATCTCTAGTGGAAAGGACGAAACACAGAACACCTACCAACTGG12.4390243902439024No Hit
ATACACGATCTCTACACGAAAGGACGAAACACAGAACAACTACCAACTCC12.4390243902439024No Hit
ATACACTATCTCTACTGGAAAGGACGAAACACCGAACACCTACCAACTCG12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)