FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs9_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs9_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6713359
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1046741.5591896694337364TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT124850.18597247666927985TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT76480.11392210665331617TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG185350.042.26463711
GATCGGA179650.042.2398341
ACACGTC186850.041.93649313
CACACGT187350.041.81282812
ACGTCTG187050.041.71430615
CGTCTGA187600.041.65095516
CACGTCT188850.041.43319714
TCGGAAG191500.041.3123553
AGCACAC190600.041.3088410
GAGCACA191850.041.00569
TCCAGTC188800.040.90912225
AGAGCAC192800.040.86068
GAACTCC190750.040.76708621
CAGTCAC190850.040.72388527
CACATGT192050.040.5387631
CCAGTCA190950.040.51762826
GTCTGAA193500.040.4832917
GTCACAT192950.040.36077529
ATCGGAA196650.040.3420452
AGTCACA193700.040.2723428
Produced by FastQC (version 0.11.2)