FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs8_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs8_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7315032
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT576580.7882125464386212TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG78850.040.48194511
GATCGGA77050.040.4188881
CACACGT80800.039.5316612
ACGTCTG80350.039.50610715
ACACGTC80850.039.39837613
CGTCTGA81050.039.2191916
CACGTCT81650.038.9579314
AGTTCCG81150.038.46816334
CCGTATC78600.038.1775738
GAGCACA84150.037.9586839
AGCACAC84150.037.9325410
GTTCCGT82300.037.77025235
CAGTCAC83800.037.72527
TCCAGTC83450.037.64389825
TCGGAAG84800.037.550853
GAACTCC84050.037.50527221
AGAGCAC85500.037.3081328
CCAGTCA84750.037.3016126
TATCTCG76800.037.26693341
GTCACAG85200.037.0020729
Produced by FastQC (version 0.11.2)