FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs7_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs7_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9118516
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1023161.1220685471188512TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG123200.041.30204411
CACACGT125000.040.7948412
ACACGTC125150.040.745513
ACGTCTG124700.040.679515
CACGTCT125150.040.638714
GATCGGA121850.040.4565351
CGTCTGA126700.040.05494716
AGCACAC129400.039.47634510
GAGCACA130500.039.2110259
CACAGTC128350.039.16510831
CAGTCAC128400.039.1155927
TCCAGTC128650.039.00409325
GTCACAG129550.038.83629229
GAACTCC130750.038.6291721
CCAGTCA130100.038.536226
CTCGTAT125100.038.51260844
AGAGCAC133100.038.4289638
AGTCACA131850.038.24225228
TCGGAAG133850.038.2070433
ATCTCGT126200.037.96814742
Produced by FastQC (version 0.11.2)