FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs6_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs6_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7051794
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC303400.43024512627566835TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG41500.038.53800611
ACACGTC42700.037.29908813
CACACGT43350.036.89231512
CGTATGC41950.036.60381744
GATCGGA42400.036.4386331
CACGTCT44200.036.0330314
ACGTCTG44550.035.7499415
AGCACAC46050.034.92156610
GAACTCC46400.034.37197521
CGTCTGA46400.034.32456616
CAGTCAC46450.034.28907827
TCCAGTC47700.033.20533425
GAGCACA48350.033.169369
CCAGTCA48000.032.86054226
GTCACCT48200.032.77029429
AGAGCAC49600.032.599798
CTCGTAT43050.032.2960942
AGTCACC50100.031.92269928
TCGTATG47700.031.8681243
TCTCGTA43300.031.80478541
Produced by FastQC (version 0.11.2)