FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs5_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs5_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7352059
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC257380.3500788010542353TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG46850.037.98711411
GATCGGA46650.037.175611
ACACGTC48300.037.0281413
ACGTCTG48600.036.70879415
CACGTCT49000.036.49867214
CACACGT49100.036.42483512
CGTCTGA49700.035.71952416
CATCTCG39950.034.748339
TCCAGTC51100.034.39721325
AGCACAC52300.034.23892210
GAGCACA52650.034.178689
GAACTCC51950.034.17247821
CAGTCAC52700.033.77147727
CCAGTCA52450.033.68009626
AGAGCAC54150.033.475888
TCGGAAG53900.033.1925773
AGTCACC53150.033.11302628
CGTATGC43150.033.0884244
CCAGATC54450.032.20123733
CTCGTAT40700.032.16160242
Produced by FastQC (version 0.11.2)