FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs4_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs4_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6758043
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC328000.4853476072880862TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC38450.039.7082429
GCACACG40150.038.7378411
AGTCACG40000.038.44426328
TCACGCC40700.037.72928630
ACACGTC41800.037.1020813
CACACGT42750.036.32931512
CACGTCT42900.036.09919714
GATCGGA41800.035.962911
ACGTCTG43150.035.94103215
CGTATGC42100.035.84623344
CACGCCA43150.035.58627731
CGTCTGA43750.035.54868716
AGCACAC44450.035.0399110
TATCTCG43950.034.18794339
TCGTATG45200.033.53351643
GAGCACA46800.033.5154579
CTCGTAT44950.033.42761242
CAGTCAC46300.033.4032527
TCCAGTC46400.033.23520325
CCAGTCA46700.033.21086526
Produced by FastQC (version 0.11.2)