FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs3_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs3_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8154970
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC552550.6775622718415886TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC174350.21379600415452174TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG100450.041.12951711
ACGTCTG102400.040.3008515
ACACGTC102800.040.23037313
CGTCTGA102850.040.10313416
GTCACAC102050.039.94597629
CACACGT104200.039.77478412
CACGTCT104050.039.72519314
GATCGGA100400.039.544061
AGCACAC106250.038.98785410
TCCAGTC104800.038.9590825
CACACAG105200.038.9176531
CAGTCAC105600.038.7276227
CCAGTCA105600.038.6433426
GAGCACA108300.038.351429
GAACTCC106750.038.2464821
CACAGTG107850.038.04345733
CGTATGC97750.037.8999544
AGAGCAC110100.037.8445438
TCGGAAG110150.037.7980843
GATCTCG72600.037.69661739
Produced by FastQC (version 0.11.2)