FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs2_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs2_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6514071
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC232180.35642841473481024TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG33150.037.29527311
CACACGT34250.036.22537212
ACACGTC34850.035.28580513
ACGTCTG35050.035.0841915
CGTATGC34550.034.76660544
GATCGGA34650.034.741761
CACGTCT35650.034.370314
AGCACAC36300.033.99856610
ACTGACC35650.033.9404432
CGTCTGA36750.033.3415316
GAACTCC36750.033.16169721
TCGTATG37500.031.9138143
TCGGAAG39300.031.5811253
CAGTCAC38650.031.53343627
TCCAGTC38650.031.4183925
CTCGTAT35450.031.33955242
CTGACCA38800.031.1844933
CACTGAC39100.031.17027731
GAGCACA40050.030.9802069
CCAGTCA39500.030.79846226
Produced by FastQC (version 0.11.2)