FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs24_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs24_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199143
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG7550.018.3566511
ACACGTC7750.017.03103313
CACACGT8200.016.90122412
CACGTCT8100.016.56655114
ACGTCTG8700.016.1825915
CGTCTGA8500.016.0457516
GATCGGA8650.016.0285991
TCCAGTC10650.012.80659125
TATCTCG9800.012.79598241
AGCACAC11400.012.73629410
GAACTCC11450.012.29582221
CAGTCAC10800.012.22193127
GAGCACA12300.011.9831099
CCAGTCA11450.011.911921526
TCGGAAG12250.011.8554013
CTCGTAT11150.011.64120344
AGTCACA12150.011.22617828
AGAGCAC13350.011.2054028
GTCACAT12750.010.87053829
ACTCCAG13350.010.21639523
Produced by FastQC (version 0.11.2)