FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs23_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs23_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8014269
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG9400.013.80771111
ACGTCTG11250.012.31897815
CACGTCT11300.012.06979514
GATCGGA12000.011.0043051
CACACGT12200.010.81904512
ACACGTC12550.010.69260713
GTCACAC12400.010.64514229
CGTCTGA13200.010.16582316
AGCACAC14950.09.12321710
CAGTCAC14250.09.10875527
CACACTG15450.08.68590531
TATCTCG14700.08.68009441
CCAGTCA15600.08.60238726
AGGGGGG9750.08.5777151
CTCGTAT17550.08.27338644
GAACTCC15900.08.16284521
TCCAGTC16150.07.900470725
GAGCACA17900.07.61971769
AGTCACA18900.07.566123528
ATCTCGT18850.07.46934242
Produced by FastQC (version 0.11.2)