FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs22_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs22_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11350468
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG15500.022.99219111
CACGAGT15850.022.34627231
AGTCACG16650.021.404828
ACGAGTG17150.020.7811232
ACACGTC17700.020.63127713
CGAGTGG17900.019.78697633
GTCACGA18050.019.62288729
ACGTCTG18500.019.50103815
CACACGT19100.019.34938612
CACGTCT20250.018.46747414
TCACGAG18950.018.45874630
GATCGGA19600.018.4148641
CGTCTGA21050.016.92966816
AGCACAC22350.016.53600910
CAGTCAC23300.015.39012427
TATCTCG25000.014.5199141
TCCAGTC24850.014.42947725
GAACTCC27150.013.85516821
GAGTGGA26850.013.76497534
GAGCACA26950.013.7135359
Produced by FastQC (version 0.11.2)