FastQCFastQC Report
Wed 15 Nov 2017
HVH7LAFXX_n01_vs21_171115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVH7LAFXX_n01_vs21_171115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7888903
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG8800.015.24936833
GCACACG10000.014.73929511
CGTACGT10050.014.0092834
GATCGGA11300.013.05032351
ACACGTC11650.012.27385813
CACACGT12500.011.96735312
CACGTCT12750.011.38711414
ACGTCTG13750.010.23899215
CGTCTGA13850.010.00623516
AGCACAC15900.09.68513310
GTACGTA15400.09.57097135
GAGCACA16100.09.5647599
ACCGTAC15650.09.55901132
TCCAGTC16450.09.49450625
CAGTCAC15700.09.24808327
GTCACCG17050.09.16096929
TCGGAAG17600.08.8780083
AGAGCAC17150.08.8510018
CACCGTA17300.08.77425931
AGTCACC17150.08.72272528
Produced by FastQC (version 0.11.2)